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Which of the following is used to determine the appropriate stringency (Wallace rule)?

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Which of the following is used to determine the appropriate stringency (Wallace rule)? Answer: Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

Question context

This question is part of 🧬 Biochemistry within the broader 🧬 Medical Science subject and appears in the DemoPaper-Pakistan preparation collection. Reviewing the answer together with nearby questions helps reinforce the underlying concept and identify how it may appear in competitive exams.