The total number of ATP used during glycolysis is?

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The total number of ATP used during glycolysis is? Answer: 2

During the energy investment phase of glycolysis, 2 ATP molecules are consumed to phosphorylate glucose and its intermediates. Later, 4 ATP molecules are produced, resulting in a net gain of 2 ATP per glucose molecule.

Question context

This question is part of 🧬 Biochemistry within the broader 🧬 Medical Science subject and appears in the PPSC Punjab Public Service Commission preparation collection. Reviewing the answer together with nearby questions helps reinforce the underlying concept and identify how it may appear in competitive exams.