🧬 Biochemistry Solved MCQs with Answers

Practice 1490 approved 🧬 Biochemistry questions with verified answers from the 🧬 Medical Science subject for Pakistan exams.

1490 solved MCQs

Deficiency of which element causes Goitre disease?Answer: Iodine What is important for strongness of the teeth?Answer: calcium HPLC stands forAnswer: Both (a) and (b) The eluent strength is a measure ofAnswer: Solvent adsorption energy HPLC methods includeAnswer: All of the above Column efficiency is measured in terms of number of plates which isAnswer: Inversely related to the square of the peak width For a typical adsorbent such as silica gel, the most popular pore diameters areAnswer: 60 and 100 A° An isocratic elution in HPLC is one in which the composition of the solventAnswer: Remains constant In reversed phase HPLC, there is aAnswer: Polar solvent/non-polar column Which of the following statements is true for a refractive index detector in HPLC?Answer: It does not respond to many solutes A gradient elution in HPLC is one in which the composition of the solventAnswer: Is changed continuously or in a series of steps An eluotropic seriesAnswer: Ranks solvents by their relative abilities to displace solutes from a given absorbent Dwell volume is defined asAnswer: The volume of the column between the point at which solvents are mixed and the beginning of the column Void volume refers to theAnswer: Total volume of eluent in the column the remainder being taken up by the packing material In normal phase HPLC, there is aAnswer: Non polar solvent/polar column Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?Answer: All of the above Which can be used as a mobile phase in HPLC applications?Answer: Any compound with solubility in liquid Which of the following is not used for detection in GC?Answer: NMR Which of these effects result from slow injection of a large sample volume?Answer: Decreased resolution The GC trace obtained after an experiment is called aAnswer: chromatogram Which of the following detectors give concentration-dependent signals?Answer: All of the above What useful information can be found from a Van Deemter plot?Answer: Optimum mobile phase flow rate What is the typical internal diameter of fused silica capillary columns?Answer: 0.2-0.3 mm Resolution is proportional to theAnswer: Square root of the number of theoretical plates in a column Derivatisation of a sample is carried out toAnswer: All of the above Which of the statements is correct?Answer: All of the above Which of the statements is correct?Answer: All of the above In column switching chromatographyAnswer: Compounds trapped on one column are eluted to another column A retention gap is placed between the injector and the front of the column toAnswer: retain contaminants and prevent them from reaching the column Which of the following detectors give mass flow-dependent signals?Answer: Field ionisation detector