🧬 Biochemistry Solved MCQs with Answers

Practice 1490 approved 🧬 Biochemistry questions with verified answers from the 🧬 Medical Science subject for Pakistan exams.

1490 solved MCQs

Transformation meansAnswer: Acquiring DNA from the bacterial cell environment Restriction mapsAnswer: Allows comparison between DNA molecules without the need to determine nucleotide sequence Which of the following is correct?Answer: All of the above A PCR cycle consists ofAnswer: three steps, denaturation, primer annealing and elongation How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?Answer: Four ds DNA molecule PCR can be used inAnswer: All of the above PCR (Polymerase Chain Reaction) can make multiple copies ofAnswer: DNA PCR is an abbreviation forAnswer: Polymerase Chain Reaction PCR amplifies millions of copies ofAnswer: A short fragment of DNA Southern blotting involvesAnswer: Electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation Bactriophages absorb to a bacterial surface and inject the phage DNA through theAnswer: Cell wall into cytosol In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise toAnswer: 16 single strands of DNA Additional mono saccharides are added in Golgi to produceAnswer: Either high mannose type oligosaccharide or a complex type The PCR, polymerase chain reaction is becoming the method of choice forAnswer: Screening gene Northern blotting isAnswer: Analogous to southern blotting What technique can be used to determine the murderer who left blood with the victim?Answer: PCR amplification DNA fragments in a restriction digest can be separated by electrophoresis inAnswer: Both (a) and (b) Reverse transcripase from HIV does notAnswer: Has a 3'- 5' exonuclease activity Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?Answer: Asn and Gln Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.Answer: 5' to 3' direction The type II restriction endonucleases, bind to DNA sitesAnswer: All of the above The enzyme that joins DNA cuts is calledAnswer: Ligase Cleavage of DNA by EcoRI endonuclease results in the formation ofAnswer: Two 3'-OH ends and two 5'-phosphate ends Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?Answer: Lys and Arg Beta pleated sheets are examples of protein'sAnswer: Secondary structure BRACl, an inherited form of breast cancer, regulates cell division byAnswer: Binding to the protein RAD 51 which repairs DNA damage Metastasis involvesAnswer: All of the above Signal sequences are part of a protein thatAnswer: Transport proteins to other sites within the cell Individuals with PKU disease are mentally retarded unlessAnswer: Phenylalanine in the diet is restricted Marfan's syndrome is thought to be a mutation affectingAnswer: Collagen synthesis