🧬 Biochemistry Solved MCQs with Answers

Practice 1490 approved 🧬 Biochemistry questions with verified answers from the 🧬 Medical Science subject for Pakistan exams.

1490 solved MCQs

Chronic granulomatous disease results from a failure to perform oxidative burst. This deficiency would be most likely to interfere withAnswer: Macrophage intracellular killing of bacteria Difficulties with somatic gene therapy arise from all of the following exceptAnswer: GVHD caused by mature T cells in the transplanted cells A monoclonal antibody (mAb) specific for the 2,4-dinitrophenyl (DNP) hapten might also bindAnswer: Tyr or Phe Retinoblastoma is due to a mutation in aAnswer: tumor supressor An autoimmune disease isAnswer: Lupus If Class IIMHC is not expressed in the thymus, the resulting immune deficiencies would include all of the following except reducedAnswer: Alternative complement activation. Specific translocations are associated withAnswer: Some leukemias To treat HIV infections using drugs, the major problem is thatAnswer: The virus particles with altered (mutant) proteases arise The primary reason for AIDS, a deadly disease is that itAnswer: Destroys key components of the body's internal defense system A selective IgA deficiency would be expected to result in problems withAnswer: Mucosal pathogens Combined cellular and humoral immune deficiencies result from lack of all of the following exceptAnswer: Transporter of antigen peptides (TAP) An example of an immunodeficiency disorder isAnswer: AIDS Bone marrow given to an infant with SCID mustAnswer: Come from a donor that shares some MHC alleles with the recipient X-linked hyper IgM syndrome, resulting in high levels of serum IgM and low levels of serum IgG, is caused by a defect in CD40L expression. The specific immune event that would be prevented by a defective CD40L would beAnswer: Failure of Th2 cells to provide co-stimulation for B cell isotype switching DiGeorge's syndrome is characterized by the lack of a thymus The mouse model closest to this human disease would be aAnswer: Nude mouse Which of the Rous sarcoma virus has a homologous cellular protein?Answer: c-src Infants are most susceptible to bacterial infection due to low circulating levels of IgGAnswer: At 3-12 months of age The chemical, typically released by the body in an allergic response isAnswer: Histamine Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectivelyAnswer: 20 kb and 2000 kb DNA The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?Answer: Blue The competitive immunoassay can be usedAnswer: Both (a) and (b) Which of the following is an immunodiffusion test?Answer: All of the above In Counter immunoelectrophoresisAnswer: Electrophoresis will drive the antibody and antigen toward each other What is the ELISA test intended to measure?Answer: Antibody to HIV only Immunoaffinity chromatography can be used in biochemical applications toAnswer: Purify protein antigen Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?Answer: Linear plasmid, open circular, super coiled plasmid In Rocket Immunodiffusion the length of the rocket isAnswer: Proportional to the amount of antigen placed in each well Radial Immunodiffusion is similar toAnswer: Double-diffusion Agarose a porous matrix used in various molecular biology technique is made by repetitive units ofAnswer: Maltose What radioactive molecule is used to end-label an oligonucleotide?Answer: (gamma)-³²P-ATP